Farmers\u27 Trust in Sources of Production and Climate Information and Their Use of Technology

A regionally representative survey of 900 Inland Pacific Northwest farmers showed that farmers trust other farmers and agribusiness most for production management decisions but trust university Extension most for climate change information. Additionally, in responding to questions about use of the Internet and mobile applications for making farm management decisions, many farmers indicated that they use the Internet daily but mobile applications much less regularly to access farm-related information. These results suggest that university Extension personnel have an important role to play in informing farmers about climate change and can do so effectively by using certain digital tools alongside other more traditional avenues for information delivery

Site-specific perturbations of alpha-synuclein fibril structure by the Parkinson's disease associated mutations A53T and E46K.

PMCID: PMC3591419This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Parkinson's disease (PD) is pathologically characterized by the presence of Lewy bodies (LBs) in dopaminergic neurons of the substantia nigra. These intracellular inclusions are largely composed of misfolded α-synuclein (AS), a neuronal protein that is abundant in the vertebrate brain. Point mutations in AS are associated with rare, early-onset forms of PD, although aggregation of the wild-type (WT) protein is observed in the more common sporadic forms of the disease. Here, we employed multidimensional solid-state NMR experiments to assess A53T and E46K mutant fibrils, in comparison to our recent description of WT AS fibrils. We made de novo chemical shift assignments for the mutants, and used these chemical shifts to empirically determine secondary structures. We observe significant perturbations in secondary structure throughout the fibril core for the E46K fibril, while the A53T fibril exhibits more localized perturbations near the mutation site. Overall, these results demonstrate that the secondary structure of A53T has some small differences from the WT and the secondary structure of E46K has significant differences, which may alter the overall structural arrangement of the fibrils

Erythropoietin Stimulates Tumor Growth via EphB4

While recombinant human erythropoietin (rhEpo) has been widely used to treat anemia in cancer patients, concerns about its adverse effects on patient survival have emerged. A lack of correlation between expression of the canonical EpoR and rhEpo’s effects on cancer cells prompted us to consider the existence of an alternative Epo receptor. Here, we identified EphB4 as an Epo receptor that triggers downstream signaling via STAT3 and promotes rhEpo induced tumor growth and progression. In human ovarian and breast cancer samples, expression of EphB4 rather than the canonical EpoR correlated with decreased disease-specific survival in rhEpo-treated patients. These results identify EphB4 as a critical mediator of erythropoietin-induced tumor progression and further provide clinically significant dimension to the biology of erythropoietin

Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)

In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. For example, a key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process versus those that measure fl ux through the autophagy pathway (i.e., the complete process including the amount and rate of cargo sequestered and degraded). In particular, a block in macroautophagy that results in autophagosome accumulation must be differentiated from stimuli that increase autophagic activity, defi ned as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (inmost higher eukaryotes and some protists such as Dictyostelium ) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the fi eld understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. It is worth emphasizing here that lysosomal digestion is a stage of autophagy and evaluating its competence is a crucial part of the evaluation of autophagic flux, or complete autophagy. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. Along these lines, because of the potential for pleiotropic effects due to blocking autophagy through genetic manipulation it is imperative to delete or knock down more than one autophagy-related gene. In addition, some individual Atg proteins, or groups of proteins, are involved in other cellular pathways so not all Atg proteins can be used as a specific marker for an autophagic process. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field

BioEarth: Envisioning and developing a new regional earth system model to inform natural and agricultural resource management

As managers of agricultural and natural resources are confronted with uncertainties in global change impacts, the complexities associated with the interconnected cycling of nitrogen, carbon, and water present daunting management challenges. Existing models provide detailed information on specific sub-systems (e.g., land, air, water, and economics). An increasing awareness of the unintended consequences of management decisions resulting from interconnectedness of these sub-systems, however, necessitates coupled regional earth system models (EaSMs). Decision makers’ needs and priorities can be integrated into the model design and development processes to enhance decision-making relevance and “usability” of EaSMs. BioEarth is a research initiative currently under development with a focus on the U.S. Pacific Northwest region that explores the coupling of multiple stand-alone EaSMs to generate usable information for resource decision-making. Direct engagement between model developers and non-academic stakeholders involved in resource and environmental management decisions throughout the model development process is a critical component of this effort. BioEarth utilizes a bottom-up approach for its land surface model that preserves fine spatial-scale sensitivities and lateral hydrologic connectivity, which makes it unique among many regional EaSMs. This paper describes the BioEarth initiative and highlights opportunities and challenges associated with coupling multiple stand-alone models to generate usable information for agricultural and natural resource decision-making

Comparison of Greenhouse Gas Offset Quantification Protocols for Nitrogen Management in Dryland Wheat Cropping Systems of the Pacific Northwest

In the carbon market, greenhouse gas (GHG) offset protocols need to ensure that emission reductions are of high quality, quantifiable, and real. Lack of consistency across protocols for quantifying emission reductions compromise the credibility of offsets generated. Thus, protocol quantification methodologies need to be periodically reviewed to ensure emission offsets are credited accurately and updated to support practical climate policy solutions. Current GHG emission offset credits generated by agricultural nitrogen (N) management activities are based on reducing the annual N fertilizer application rate for a given crop without reducing yield. We performed a “road test” of agricultural N management protocols to evaluate differences among protocol components and quantify nitrous oxide (N2O) emission reductions under sample projects relevant to N management in dryland, wheat-based cropping systems of the inland Pacific Northwest (iPNW). We evaluated five agricultural N management offset protocols applicable to North America: two methodologies of American Carbon Registry (ACR1 and ACR2), Verified Carbon Standard (VCS), Climate Action Reserve (CAR), and Alberta Offset Credit System (Alberta). We found that only two protocols, ACR2 and VCS, were suitable for this study, in which four sample projects were developed representing feasible N fertilizer rate reduction activities. The ACR2 and VCS protocols had identical baseline and project emission quantification methodologies resulting in identical emission reduction values. Reducing N fertilizer application rate by switching to variable rate N (sample projects 1–3) or split N application (sample project 4) management resulted in a N2O emission reduction ranging from 0.07 to 0.16, and 0.26 Mg CO2e ha−1, respectively. Across the range of C prices considered ($5,$10, and $50 per metric ton of CO2 equivalent), we concluded that the N2O emission offset payment alone ($0.35–$13.0 ha−1) was unlikely to encourage a change in fertilizer N management; however, the fertilizer cost savings from adopting variable or split N management would incentivize adopting these practices. Therefore, the monetary incentive of adopting agricultural N management BMPs for reducing N2O emission should be tied to other co-benefits and existing conservation programs to encourage N rate reductions that do not limit yield, crop quality, or economic stability

Using participatory risk analysis to develop a song about malaria for young children in Limpopo Province, South Africa

Abstract Background In 2015, malaria infected over 212 million people and killed over 429,000 individuals, mostly children under 5 years of age, with 90% of malaria cases occurring in sub-Saharan Africa. The aim was to develop an age and culturally appropriate song for Tshivenda-speaking children under 5 years of age to decrease the risk of malaria in Limpopo Province, South Africa. Methods Document review was used to identify appropriate disease determinants to decrease risk in children < 5 years old in the study area. These were used to develop lyrics and music for a song about malaria in line with the principles of participatory risk analysis. The age and cultural appropriateness of the song as well as disease determinants chosen were reviewed using a modified Delphi technique, by 10 purposively selected experts in malaria (4), Vhavenda music (3) and early childhood education (3). Thereafter, the song was translated into Tshivenda and reviewed by two focus groups living in the study area, one including female caregivers and pre-school teachers (n = 7) and a second comprising of male community based malaria control personnel (n = 5). Results The experts surveyed and both focus groups strongly supported the inclusion of knowledge about the link between mosquitoes and malaria and that children should know the signs of malaria to facilitate early diagnosis. Although the expert group felt that bed nets should not be mentioned, both focus groups suggested the inclusion of bed nets and it was observed that community members were purchasing their own nets. Focus group members also felt that young children should not be involved in internal residual spraying initiatives. Conclusions It was concluded that although risk communication on malaria prevention and treatment in young children should be aimed at caregivers, an age and culture appropriate song about malaria could be developed to help young children protect themselves. This song focused on understanding the link between mosquitoes and malaria, preventing exposure and recognising signs of disease

Design and Use of Representative Agricultural Pathways for Integrated Assessment of Climate Change in U.S. Pacific Northwest Cereal-Based Systems

This paper presents the design and use of Representative Agricultural Pathways (RAPs) in regional integrated assessment of climate impacts. In the first part of the paper, we describe the role of pathways and scenarios in regional integrated assessment as well as the three RAPs developed for a study of dryland wheat-based systems in the U.S. Pacific Northwest. We use this example to illustrate the challenges associated with the development and implementation of RAPs, including the engagement of research team and stakeholders, the dimensionality problem in integrated assessment, incorporation of economic data, and quantification of uncertainties. In the second part, we illustrate the use of RAPs in the study of climate impacts on dryland wheat-based systems. Results show that the direct impacts of future climate projections through crop yields provide the largest source of uncertainty in the climate impact and vulnerability analysis, but the indirect impacts of climate change through price projections embedded in RAPs also play an important role in the analysis. We conclude that in addition to being an essential element in designing an integrated assessment at the regional level, the RAPs development process can facilitate stakeholder engagement and improve communication of climate impact assessments

Climate analogs can catalyze cross-regional dialogs for US specialty crop adaptation

Abstract Communication theory suggests that interactive dialog rather than information transmission is necessary for climate change action, especially for complex systems like agriculture. Climate analogs—locations whose current climate is similar to a target location’s future climate—have garnered recent interest as transmitting more relatable information; however, they have unexplored potential in facilitating meaningful dialogs, and whether the way the analogs are developed could make a difference. We developed climate context-specific analogs based on agriculturally-relevant climate metrics for US specialty crop production, and explored their potential for facilitating dialogs on climate adaptation options. Over 80% of US specialty crop counties had acceptable US analogs for the mid-twenty-first century, especially in the West and Northeast which had greater similarities in the crops produced across target-analog pairs. Western counties generally had analogs to the south, and those in other regions had them to the west. A pilot dialog of target-analog pairs showed promise in eliciting actionable adaptation insights, indicating potential value in incorporating analog-driven dialogs more broadly in climate change communication